Abstract
AbstractLipoxygenases (LOX) catalyse allylic oxidations and epoxidations of a co‐substrate in the presence of an unsaturated fatty acid containing a 1,4‐pentadiene moiety. One‐ and two‐step enzyme assays were established to verify the role or involvement of LOX in such co‐oxidations. It was shown that LOX is only involved in the formation of reactive hydroperoxides, but not in the oxidation of a co‐substrate, assuming a mechanism involving free peroxy radicals for the latter. Ten mono‐ and sesquiterpenes were used as co‐substrates and the resulting products were analysed by mass spectrometry. A semi‐preparative approach was developed using (+)‐valencene as an example, and the resulting products were isolated by preparative GC and their structures elucidated by NMR spectroscopy.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
