Abstract
This chapter aims to determine whether liposomes could be used as a model for: the screening of shark repellents, and understanding the mechanism of shark repellency by detergents. Shark repellency was tested using nonionic and ionic detergents. The latter include anionic and cationic detergents. The determination of electrical surface potential of the detergent micelles is based on the use of 4-heptadecyl-7-hydroxycoumarin. This molecule serves as a surface pH indicator at the interface between the hydrophobic and polar phases, such as at the surface of a detergent micelle, lipid vesicles, and biological membranes. When a detergent is added to a liposome suspension it divides into three phases: monomers in the aqueous solution, micelles, and a bilayer component. Increase in turbidity of liposome suspensions occurs upon liposome aggregation and/or size increase. The opposite occurs upon liposome solubilization, which is followed by major turbidity reduction.
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