Abstract
As previously demonstrated, membrane fusion and transfer of prodrug-type molecules between membranes can be studied with the octadecylrhodamine B chloride (R18) marker. We use this assay with PR8 influenza viruses and small (SUV) or large (LUV) unilamellar liposomes containing the virus receptor G D1a as interacting partners in order to analyse the effect of membrane curvature on fusion and transfer. Computer analysis revelaed no difference in the kinetics of fusion between PR8 with large or small liposomes respectively. Lipid transfer, however, is about 5 to 6 times faster from SUV than from LUV. We also tested the influence of the production method for liposomes, namely detergent dialysis, sonication and extrusion on membrane interactions. Fusion is not affected by either, whereas transfer is slower with liposomes produced by sonication.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.