Abstract
In lactation metabolic adaptations occur to ensure that the mammary gland is continuously supplied with substrates for milk production. One of these changes is that the activity of lipoprotein lipase is decreased in adipose tissue but increased in mammary gland thereby aiding in the direction of lipid to mammary gland [l-31. AAer removal of the litter lipoprotein lipase activity increases again [4,5]. The aim of this work was to determine whether the low lipoprotein lipase activity in adipose tissue in lactation could be explained by a decrease in lipoprotein lipase mRNA levels. Lipoprotein lipase activity was measured in infranates of homogenates [6] of rat parametrial adipose tissue with Intralipid as substrate [7]. The results in Table 1 show that by 10 d of lactation lipoprotein lipase activity in parametrial adipose tissue had fallen to 33% of that in adipose tissue from virgin rats. At 24h after removal of the litter, lipoprotein lipase activity was still low. However, by 48h after removal of the litter lipoprotein lipase activity had returned to values similar to those in adipose tissue from virgin rats. Total RNA was extracted [8] and LPL and a-tubulin mRNA measured following Northern blotting [9]. The results in Table 2 show that lipoprotein lipase mRNA, but not a-tubulin mRNA, was decreased in parametrial adipose tissue compared to the values in virgin rats. Lipoprotein lipase mRNA levels had not increased by 24h after removal of the litter.
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