Lipoprotein lipase mass and activity in severe hypertriglyceridemia

Abstract

To clarify the role of defective lipoprotein lipase (LPL) in hypertriglyceridemia, the LPL masses and LPL activities in post-heparin plasma (PHP) were studied in severe hypertriglyceridemias. The developed sandwich enzyme immunoassay for the LPL was sensitive from 0.5 to 20 ng/ml of LPL in human PHP. The plasma LP mass increased by heparin injection (30 USP units/kg) and was found to positively correlate with LPL activity. The mean LPL activity from PHP of normal controls was 2,960 ± 1,057 nmol/ml/h . The mean LPL masses from human pre- and 15-min post-heparin plasma from normal subjects were 25 ± 5 ng/ml and 224 ± 60 ng/ml , respectively. Thus the specific activity of LPL from PHP of normal controls was calculated to be 13.3 μmol FFA released/h/μg LPL. Among hypertriglyceridemic patients with over 1,000 mg/dl of serum triglyceride, the incidence of patients with LPL masses less than −2 standard deviations (S.D.) of those of average normal control subjects was found to be 27%. Seventy percent of patients showed specific activities within + 2 S.D. of those of average control LPL, and 30% showed significantly low specific activities less than −2 S.D. despite the fact that LPL masses were not less than −2 S.D. of the average normal controls. These results suggest that the evaluation of LPL masses in PHP would be useful for finding functionally defective LPL in patients with hypertriglyceridemia, and that up to 30% severe hypertriglyceridemias may have functionally defective LPL.