Lipoprotein lipase in rat heart—I. Characterization of tri-, di- and monoacylglycerol lipase activities in post-heparin effluents

Abstract

1. 1. The lipolytic activities that sequentially hydrolyze tri-, di- and monoacylglycerol in rat post-heparin heart effluents were examined. 2. 2. Properties of triacylglycerol lipase (TAGL) activity were typical of lipoprotein lipase. Diacylglyceorl lipase (DAGL) behaved similarly to TAGL, suggesting that both activities refer to the same catalytic entity. 3. 3. Differences, particularly in thermal stability, between TAGL and DAGL activities on one hand, and monoacylglycerol lipase (MAGL) activity on the other, may reflect different intrinsic molecular properties. 4. 4. TAGL, DAGL and MAGL activities could not be separated by physical means and appeared to belong to a single unit at the same site on the capillary wall.