Abstract
D-galactosamine (GalN) produces, in the rat, an acute hepatitis, LCAT deficiency, and plasma lipoprotein abnormalities similar to those of human liver disease. Rats were sacrificed at intervals up to 24 h after a single ip injection of GalN, 750 mg/kg. No alpha band was found by plasma lipoprotein electrophoresis 12-24 h after GalN. Plasma LCAT activity was decreased 45% at 6 h and was < 10% of normal at 24 h. Cholesteryl esters decreased to 36% of total cholesterol at 12 h (control 70%) and at 24 h after GalN were only 15% of total cholesterol. Fractionation of plasma phospholipids at 24 h revealed lecithin increased to 68% of total phospholipid (control 46%), and lysolecithin decreased to 12% (control 25%). The increase of the lecithin/lysolecithin ratio to 5.6 after GalN (control 1.8) may reflect decreased LCAT activity. Arachidonyl esters accounted for only 9% of cholesteryl esters after GalN compared to 64% in controls. Lipoproteins were isolated by sequential ultracentrifugation: VLDL (d < 1.006); L...
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