Abstract
The lipopolysaccharide (LPS) ofPseudomonas aeruginosa (ATCC 9027) exhibited gross changes in both the amount of LPS produced per cell and the composition of the LPS in response to changes in magnesium ion concentrations. Compositional variation in the LPS was detected under both batch and continuous culture conditions, and was particularly apparent as changes in the levels of heptose in the molecule. Compositional changes in the molecule were also reflected as functional alterations in the LPS. However, functionally, these alterations were counterbalanced by increased production of the lipopolysaccharide. We propose that LPS composition changed in accordance with environmental factors, and that cells responded to these changes by increasing or decreasing the amount of LPS produced.
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