Abstract
BackgroundHost cell autophagy is implicated in the control of intracellular pathogen. Escherichia coli (E.coli) is the most common organism caused single-germ enterobacterial peritonitis during peritoneal dialysis. In this study, we investigated autophagy of peritoneal mesothelial cells and its role in defense against E.coli.ResultsAutophagy in human peritoneal mesothelial cell line (HMrSV5) was induced by lipopolysaccharide (LPS) in a dose-dependent and time-dependent way, which was demonstrated by increased expression of Beclin-1 and light chain 3 (LC3)-II, the accumulation of punctate green fluorescent protein-LC3, and a higher number of monodansylcadaverine-labeled autophagic vacuoles. After incubation of HMrSV5 cells with E.coli following LPS stimulation, both the intracellular bactericidal activity and the co-localization of E.coli (K12-strain) with autophagosomes were enhanced. Conversely, blockade of autophagy with 3-methyladenine, wortmannin or Beclin-1 small-interfering RNA (siRNA) led to a significant reduction in autophagy-associated protein expression, attenuation of intracellular bactericidal activity, and reduced co-localization of E.coli with monodansylcadaverine-labeled autophagosomes. In addition, treatment of HMrSV5 cells with LPS caused a dose-dependent and time-dependent increase in Toll-like receptor 4 (TLR4) expression. Both knockdown of TLR4 with siRNA and pharmacological inhibition of TLR4 with Polymyxin B significantly decreased LPS-induced autophagy. Furthermore, TLR4 siRNA attenuated remarkably LPS-induced intracellular bactericidal activity.ConclusionsOur findings demonstrated for the first time that LPS-induced autophagy in peritoneal mesothelial cells could enhance the intracellular bactericidal activity and the co-localization of E.coli with autophagosomes. The activation of TLR4 signaling was involved in this process. These results indicate that LPS-induced autophagy may be a cell-autonomous defense mechanism triggered in peritoneal mesothelial cells in response to E.coli infection.
Highlights
Host cell autophagy is implicated in the control of intracellular pathogen
Characterization of human peritoneal mesothelial cell line (HMrSV5) in culture Confluent HMrSV5 cells exhibited multipolar with a uniform cobblestone-like appearance under the phase contrast microscope
Autophagy in HMrSV5 cells was induced in response to LPS stimulation Light chain 3 (LC3) exists in two forms, the 18 kDa cytosolic form (LC3-I), and the 16 kDa processed form
Summary
Host cell autophagy is implicated in the control of intracellular pathogen. Escherichia coli (E.coli) is the most common organism caused single-germ enterobacterial peritonitis during peritoneal dialysis. We investigated autophagy of peritoneal mesothelial cells and its role in defense against E.coli. Escherichia coli (E.coli) is the most common organism caused single-germ enterobacterial peritonitis during PD [12,13]. The induction of autophagy by LPS in peritoneal mesothelial cells (PMCs), which provides a nonadhesive and protective layer in the abdominal cavity against the invasion of foreign particles and injury [17], and the role of autophagy in the elimination of E. coli from PMCs have not been studied yet. The objective of present study was to investigate the autophagy induced by LPS in PMCs and its role in defense against E. coli. We were interested in determining whether autophagy contributes to E.coli survival or death
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.