Lipopolysaccharide が骨芽細胞の増殖と分化におよぼす影響

Abstract

Lipopolysaccharide (LPS) is one of the candidates for the alveolar bone loss accompanied with periodontal disease. The purpose of this study is to assess the effect of LPS on cell proliferation and the differentiation of osteoblastic cells in cell culture. Osteoblastic cells were prepared from rat calvaria, and the cells were cultured with LPS in the presence and the absence of ascorbic acid. After the culture, the cell proliferation was examied by scoring colony formed or estimating cell number using cell counting kit. The osteoblastic differentiation was examined by measuring ALP activity of cell lysate. The results obtained were as follows : 1. In osteoblastic cell culture at low cell density, many colonies were formed. More than half of these colonies were ALP positive from the histochemical stainning. Mineralized nodules were appeared in the culture adding ascorbic acid and β-glycerophophate. 2. LPS (0.1, 1, 10μg/ml) increased cell number of osteoblastic cells in colony assay and in the assay cell counting kit. 3. LPS greatly affected ALP activity of osteoblastic cells. The activity was also positively and negatively influenced by the addition of ascorbic acid and cell density. Thus, LPS increased osteoblastic cell proliferation and affected ALP activity of osteoblastic cells. These results suggest that LPS modulate alveolar bone loss accompanied with periodontal disease.