Abstract
Two bacterial isolates from a mixed microbial culture able to degrade wool-scour effluent were found to synthesize and produce lipolytic activity. The growth, esterase production, and wool grease degradation by these strains, a Pseudomonas aeruginosa and an Acinetobacter calcoaceticus, were compared with other characterized P. aeruginosa and A. calcoaceticus strains. Growth of all strains in minimal media containing wool-scour effluent as a carbon source resulted in the synthesis of extracellular lipolytic activity which hydrolysed p-nitrophenyl palmitate, α-naphthyl acetate, α-naphthyl palmitate, cholesteryl oleate, cetyl palmitate, stearyl laurate, and tributyrin.
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