Abstract
There is considerable uncertainty with regard to the amounts, if any, of cholesterol and fatty acids in the spinal fluid, largely because the methods available are not sufficiently refined to measure accurately the small quantities in a few cubic centimeters of fluid. The large amounts (80 to 120 cc.) of cerebrospinal fluid removed in preparing patients for encephalographic examination have made it possible to obtain sufficient lipoid material for study. The proteins were determined by several methods in order to obtain data on the ratio between cholesterol and protein in spinal fluid. These results proved interesting in connection with previous studies on composition and formation of transudates.<sup>1</sup> The early attempts of Mott<sup>2</sup>and of Mestrezat<sup>3</sup>to recover cholesterol from the cerebrospinal fluid of persons free from obvious meningeal disorders yielded nothing, or only very slight traces. Subsequent workers have reported finding amounts that ranged from zero to
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