Lipocyte hyperplasia and sexual maturation of Japanese quail (Coturnix coturnix japonica)

Abstract

1. Hyperplastic growth of adipose tissue in Japanese quail (Coturnix coturnix japonica) was examined in relation to sexual maturity to determine whether fat cell number is fixed in mature individuals of this species. 2. Total DNA concentrations in the lipid (lipocyte) and non-lipid (stroma) fractions of collagenase (EC 3.4.24.3)-digested abdominal (retroperitoneal) fat depots were determined on a chronological-age basis from 28 to 240 d of age. The in vivo incorporation of [methyl-3H]thymidine into DNA of both the lipocyte and stroma fractions of abdominal fat from both ad lib. and restricted-fed (75% of ad lib.) females was also examined at ages before and subsequent to sexual maturity. 3. In both males and females, significant increases in abdominal fat weights at ages beyond sexual maturity were associated with increased lipocyte DNA. Regardless of the feeding regimen and stage of maturity, substantial radioactivity was recovered from both the stromal and lipocyte fractions of abdominal fat when female quail were examined 24 h after the administration of tritiated thymidine. When examined 5 d post-injection, the majority of the radioactivity was contained in the lipid fraction of collagenase-digested adipose tissue. 4. Both the total DNA content of adipose tissue and the incorporation of tritiated thymidine into adipose tissue indicated that lipocyte hyperplasia contributes to postmaturational increases in fat deposition.