Abstract
Summary Cottonseed oil is effectively oxidized with a mixture of perchloric and nitric acids in connection with the determination of phosphorus by the Fisk and Subbarow method. The development of the molybdenum blue in the procedure is independent of the acid content in the reducing medium up to 0.5 normal. The presence of perchloric acid does not interfere with the development of the molybdenum blue. The phosphorus content of cottonseed extracts depends on the nature of the solvent. The quantities obtained varied from 0.0071 to 0.117%. A phospholipid containing 3.73% phosphorus was isolated from cottonseed.
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