Lipids by Yarrowia lipolytica Strains Cultivated on Glucose in Batch Cultures.

Erdem Carsanba,Huseyin Erten,Patrick Fickers,Seraphim Papanikolaou

doi:10.3390/microorganisms8071054

Abstract

Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have many advantages such as wide substrate range usage and robustness to extreme conditions, while under several culture conditions it can produce high lipid productivity. Based on this assumption, in this study, 12 different Yarrowia lipolytica strains were used to investigate microbial lipid production using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve wild-type or mutant strains of Yarrowia lipolytica which were newly isolated or belonged to official culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917 and Po1dL.

Highlights

Microbial lipids or single-cell oils (SCO) contain triacylglycerols (TAGs), glycolipids, phospholipids and sterylesters
As a potent oleaginous yeast, Yarrowia lipolytica can accumulate lipids to more than 20% of its dry biomass, consisting mostly of unsaturated fatty acids, as in plant oils, and this is of industrial importance in food biotechnology
Of the 12 strains, ten Y. lipolytica strains exhibited an oleaginous property, producing more than 20% (g lipid/g dry cell weight) of the lipid in dry cell weight value—two strains did not exhibit this property (K57 and CBS 6303)

Summary

Introduction

Microbial lipids or single-cell oils (SCO) contain triacylglycerols (TAGs) (lipids of energy reserve), glycolipids (lipids of membrane structure), phospholipids and sterylesters. As a potent oleaginous yeast, Yarrowia lipolytica can accumulate lipids to more than 20% of its dry biomass, consisting mostly of unsaturated fatty acids, as in plant oils, and this is of industrial importance in food biotechnology. It has been widely used in the production of lipids and lipid-derived compounds such as biodiesel, edible oils or dicarboxylic acids, which are used as building blocks for polymers synthesis [8]. Y. lipolytica has many advantages over other oleaginous microorganisms: principally, its high productivity, easier cultivation, wide range of substrate usage (unrefined feedstock and industrial residues) and good tolerance to high substrate concentrations, salt, metal ions and difficult environmental factors such as low and high pH (from 2.5 to 9.0) and a wide range of temperatures (from 18 ◦C to 32 ◦C) [6,9,10,11,12]

Methods

Results

Conclusion