Abstract
Contrary to previous reports, ornithine amide lipids are produced by some strains of Ralstonia eutropha and Ralstonia pickettii under some growth conditions, thus eroding one of the characteristic differences from Burkholderia spp. The proportion of phosphatidylethanolamine containing 2-hydroxy acids also varies with growth conditions, but not significantly with growth temperature (30 or 37°C) for shake-flask cultures, unlike chemostat cultures of Burkholderia cepacia. Several fatty acid profiles were represented among the isolated lipopolysaccharides (LPS). Burkholderia spp. typically had 14:0, 3-OH-14:0 and 3-OH-16:0 as major components, but in two strains of B. cepacia the LPS was relatively rich in 12:0 (rather than 14:0) or contained 2-OH-14:0 (but little 3-OH-16:0). In two strains each of R. eutropha and R. pickettii, the LPS contained mainly 14:0 and 3-OH-14:0 with some 2-OH-14:0. In another strain of R. pickettii the LPS contained 2-OH-16:0 but not 2-OH-14:0. The data may have chemotaxonomic potential.
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