Lipidomic Profiling Reveals Distinct Differences in Sphingolipids Metabolic Pathway between Healthy Apis cerana cerana larvae and Chinese Sacbrood Disease.

Linling Wang,Dunyuan Huang,Jinshan Xu,Zeyang Zhou,Yan Li,Zhengang Ma,Xiaoqun Dang,Chengcheng Wang,Xiaodong Fan,Xiaoqing Li,Zhi Li

doi:10.3390/insects12080703

Linling Wang, Dunyuan Huang + Show 9 more

Open Access

https://doi.org/10.3390/insects12080703

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Journal: Insects	Publication Date: Aug 5, 2021
Citations: 4	License type: CC BY 4.0

Affiliation: Chongqing Normal University, Southwest University

Abstract

Chinese sacbrood disease (CSD), which is caused by Chinese sacbrood virus (CSBV), is a major viral disease in Apis cerana cerana larvae. Analysis of lipid composition is critical to the study of CSBV replication. The host lipidome profiling during CSBV infection has not been conducted. This paper identified the lipidome of the CSBV-larvae interaction through high-resolution mass spectrometry. A total of 2164 lipids were detected and divided into 20 categories. Comparison of lipidome between healthy and CSBV infected-larvae showed that 266 lipid species were altered by CSBV infection. Furthermore, qRT-PCR showed that various sphingolipid enzymes and the contents of sphingolipids in the larvae were increased, indicating that sphingolipids may be important for CSBV infection. Importantly, Cer (d14:1 + hO/21:0 + O), DG (41:0e), PE (18:0e/18:3), SM (d20:0/19:1), SM (d37:1), TG (16:0/18:1/18:3), TG (18:1/20:4/21:0) and TG (43:7) were significantly altered in both CSBV_24 h vs. CK_24 h and CSBV_48 h vs. CK_48 h. Moreover, TG (39:6), which was increased by more than 10-fold, could be used as a biomarker for the early detection of CSD. This study provides evidence that global lipidome homeostasis in A. c. cerana larvae is remodeled after CSBV infection. Detailed studies in the future may improve the understanding of the relationship between the sphingolipid pathway and CSBV replication.

Highlights

Apis mellifera and Apis cerana cerana, which are required for agricultural production and nutrient-rich beehive products, are increasingly grown in China [1]
The peaks extracted from all the experimental samples and quality control samples (QC) samples were analyzed by PCA
To gain insight into the lipid metabolic reprogramming of the larvae during CSBVTo gain insight into the lipid metabolic reprogramming of the larvae during infection, we first analyzed the glycerolipids (GP) profile during viral replication in comCSBV-infection, we to first analyzedlarvae, the glycerolipids profile during viral replication parison uninfected which were(GP)

Summary

Introduction

Apis mellifera and Apis cerana cerana, which are required for agricultural production and nutrient-rich beehive products, are increasingly grown in China [1]. Infection by SBV is lethal to honeybee larvae, which results in entire colony collapse for A. c. Serum lipidome analysis after Zika virus (ZIKV) infection demonstrated an increase in several phosphatidylethanolamine (PE) lipid species, suggesting a link between ZIKV life cycle and peroxisomes [19]. DENV revealed that glycerophospholipids, sphingolipids and fatty acyls were coincident with the kinetics of viral replication [20,21]. These data demonstrate that lipids play critical roles in viral infection. Identification and analysis of lipid components of the host are important for the investigation of the molecular mechanism of CSBV replication. QRT-PCR analysis revealed that sphingolipid was the most perturbed pathway after CSBV infection

Sample Collection

Lipidomics

Quantitative Real-Time PCR

Glycerolipids Showed Maximal Remodeling after CSBV Infection

Lipid species ofwere

CSBV Infection Causes an Accumulation of Sphingolipids

Biomarkers Screening of the CSBV-Infected Larvae

Ceramide

Pathway