Abstract
1. 1. Adult male Holtzman rats were each given a single injection of P 32 as NaH 2P 32O 4 at 1, 3, and 18 days after partial hepatectomy, and were sacrificed 1 hr. later. Six hours prior to sacrifice, unoperated controls and 3-day hepatectomized rats received 0.03 mg. colchicine/100 g. body weight by subcutaneous injection. Livers were removed and the radioactivity and P in the lecithin, cephalin, and sphingomyelin fractions, as well as di-, tri-, tetra-, and pentaenoic acids were determined. 2. 2. The high P 32 uptake by the phospholipide fraction coincident with mitosis occurred in all three types of phospholipide isolated. It was not abolished by colchicine, a metaphase inhibitor, suggesting that it is associated with either interphase or prophase, but not with anaphase or telophase. 3. 3. There was an increase in the concentration of trienoic and a decrease in tetraenoic acid coincident with the period of hypertrophy (premitosis) in regenerating liver. There was an increase in dienoic acid coincident with the period of high mitotic activity. Blocking mitosis in metaphase with colchicine did not influence the level of dienoic acid.
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