Lipid transport mechanism in the fish-V. Isolation and characterization of carp plasma lipoproteins.

Abstract

VLDL (d<1.006g/ml), LDL1(d 1.006-1.063g/ml), LDL2 (d 1.063-1.125g/ml), and HDL (d 1.125-1.21g/ml) were isolated by sequential ultracentrifugation from carp plasma obtained at 8h after feeding and purified by gel-filtration column chromatographies. Carp VLDL, LDL2, and HDL were all detected as a single band, but LDL1 consisted of three bands with lipid staining by polyacrylamide gel electrophoresis. The major lipid components of VLDL and LDL1 were triglycerides. Moreover, both VLDL and LDL1 contained human apo B-, apo A-II-, and apo C- like proteins. Carp LDL2 contained relatively high levels of sterol esters in addition to trig-lycerides, and contained exclusively human apo B-like protein. Carp HDL, which was the pre-dominant class in all lipoproteins, contained phospholipids as the major lipid components and human apo A-I-and A-II-like proteins as the major apoprotein components.