Lipid Targeting of Synaptotagmin I C2 Domains on Asymmetric Two-Phase Planar Bilayers

Abstract

We showed previously that cholesterol-rich liquid-ordered domains with lipid compositions typically found in the outer leaflet of plasma membranes could induce liquid-ordered domains in adjacent regions of asymmetric lipid bilayers with apposed leaflets composed of typical inner leaflet lipid mixtures (Biophys.J. 91:3313-26 [2006]). The lipid requirements for this transbilayer coupling in asymmetric cholesterol-rich two-phase lipid bilayers were further investigated and found to be roughly correlated with their chain-melting phase transition temperatures (Biochemistry 47: 2190-8 [2008]).