Abstract
Whole homogenates of rat submandibular salivary gland were fractionated by differential centrifugation into several particulate species, including mitochondria, microsomes and plasma membrane. Fatty acid CoA synthetase (E.C. 6.2.1.3) activity was present in relatively high levels in all fractions except plasma membrane. Palmitic acid, either as the free acid in the presence of ATP, CoA and Mg 2+, or as palmityl-CoA, was readily incorporated into the phospholipid and triglycerides of all fractions although the microsomal preparations exhibited the highest activity. Carnitine-palmityltransferase and lysolecithin-palmityltransferase activites were found in all fractions. The formation of phosphatidyl inositol from [ 14C]-inositol was observed in all fractions, but the highest incorporation occurred with microsomes. Mitochondrial preparations had the ability to form another carnitine-containing lipid besides the O-acylcarnitine ester. The formation of this lipid appears to be characteristic of mitochondria.
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