Abstract
Histamine release was induced from perfused cat paws and isolated rat mast cells with compound 48 80 , and from sensitized guinea pig lungs with antigen. Concomitantly occurring biologically active substances were separated by various Chromatographic procedures (silicic acid column, silicic acid impregnated paper, etc.). From all the species several spasmogenic principles were obtained. Chemical and biological properties indicated the presence of unsaturated fatty acids, phosphatidyl and phosphatidal choline and an SRS principle. The SRS principles from the three species seemed to be identical or very closely related.
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