Lipid regulation in SZ95 sebocytes by active and inactive phospholipases A2fromBothrops moojenivenom

Abstract

The scientific background for this work is based on the fact that sebocytes reduce lipid production during the skin ageing process. The relationships between ageing effects and lipid reduction on a molecular level and ways to influence them are not yet fully identified. For this reason we investigated lipid stimulation in SZ95 sebocytes by Bothrops moojeni snake venom gel filtration fractions (Botmo GF). Several Botmo GFs increased lipid synthesis in SZ95 sebocytes without apparent toxic or apoptotic effects in applied concentrations. Botmo GF 11 was further purified by RP-HPLC. Fraction Botmo GF 11-117 showed phospholipase A2 (PLA2) activity, whereas fraction Botmo GF 11-101 did not. At a concentration of 1 μg/ml Botmo GF 11-101 enhanced neutral lipid synthesis by 150% and Botmo GF 11-117 by 310% compared to untreated SZ95 sebocytes. It was supposed that sebum production is stimulated via a PPAR agonistic mechanism of free fatty acids that were released by PLA2 activity. However, the present data surprisingly indicate that lipid synthesis stimulation by Botmo GF 11-101 and Botmo GF 11-117 is independent of PLA2 enzymatic activity in Botmo GF 11 subfractions. Thus, it is suspected that both Bothrops moojeni fractions act via different mechanisms on lipid stimulation in SZ95 sebocytes.Although the exact mode of actions are not yet identified, the Botmo GF 11-101 and Botmo GF 11-117 might be interesting tools for the investigation of sebocyte lipogenesis and may be helpful to the development of therapeutic concepts for the treatment of age-related skin dryness.