Lipid Profiles of the Heads of Four Shrimp Species by UPLC-Q-Exactive Orbitrap/MS and Their Cardiovascular Activities.

Yongqiang Zhu,Peihai Li,Ronghua Meng,Hongbin Zhai,Shanshan Zhang,Xiaobin Li,Lizheng Wang,Kechun Liu,Yuezi Qiu,Xuanming Zhang,Houwen Lin

doi:10.3390/molecules27020350

Abstract

Lipids are key factors in nutrition, structural function, metabolic features, and other biological functions. In this study, the lipids from the heads of four species of shrimp (Fenneropenaeus chinensis (FC), Penaeus japonicus (PJ), Penaeus vannamei (PV), and Procambarus clarkia (PCC)) were compared and characterized based on UPLC–Q–Exactive Orbitrap/MS. We compared the differences in lipid composition of four kinds of shrimp head using multivariate analysis. In addition, a zebrafish model was used to evaluate pro-angiogenic, anti-inflammatory, anti-thrombotic, and cardioprotective activities of the shrimp head lipids. The lipids from the four kinds of shrimp head had different degrees of pro-angiogenic activities, and the activities of PCC and PJ shrimp lipids were more significant than those of the other two species. Four lipid groups displayed strong anti-inflammatory activities. For antithrombotic activity, only PCC (25 μg/mL) and PV (100 μg/mL) groups showed obvious activity. In terms of cardioprotective activity, the four kinds of lipid groups significantly increased the zebrafish heart rhythms. The heart distances were shortened, except for those of the FC (100 μg/mL) and PJ (25 μg/mL) groups. Our comprehensive lipidomics analysis and bioactivity study of lipids from different sources could provide a basis for the better utilization of shrimp.

Highlights

GP was significantly less in PCC than that in the other three kinds of shrimp heads, suggesting that marine shrimp are richer in phospholipids than land shrimp
We studied the cardiac protective activities of shrimp head lipids via the zebrafish heart injury model and evaluated the effect by measuring the heart rate and sinus venosus and bulb arterial (SV-BA) distance of zebrafish [37]
Study on of ported that shrimp heads are rich in lipid components

Summary

Introduction

Endow them with a variety of important biological functions, including energy conversion, material transport, cell development and differentiation, identification and delivery information, and apoptosis [3,4,5,6]. Lipids have a variety of biological activities, such as cardiovascular protective activity [7,8], pro-angiogenic activity [9], hepatic protective activity [10], and anti-inflammatory activity [11,12]. The identification of lipids using a high-throughput and sensitive mass spectrometry method is crucial for elucidating the role of lipids in cell function and disease treatment

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Conclusion