Abstract
Levels of lipid peroxidation in hepatopancreas (HP), gill (G), and hemolymph (HYM) of stone violaceous crab Platyxanthus orbignyi (Milne Edwards and Lucas (1843)) were performed to examine the effect of short exposure to air. After four hours animals were collected, 14 from exposure to air and 10 from seawater were dissected and their lipid peroxidation (LPO) levels were evaluated using the ferrous oxidation-xylenol orange (FOX) method, in gill, hepatopancreas, and hemolymph. The total mortality of those crabs was evaluated after seven hours at 22 ± 1 °C on exposure to air conditions. Levels of LPO in hepatopancreas (female/male = 4.68 ± 1.60/5.12 ± 1.59 Eq-H2O2/g wet tissue) and hemolymph (female/male = 1.48 ± 1.42/1.28 ± 1.06 Eq-H2O2/g wet tissue) displayed no significant differences, in contrast, gills displayed significant differences (male/female = 5.63 ± 0.83/4.63 ± 0.44 Eq-H2O2/g wet tissue, p < 0.05). The results showed that air exposure in the short term in this study induces a different response in oxidative stress levels and damage could be accompanied by accumulation of peroxide lipids (LOOH). These results suggest that different organs can show different responses to oxidative stress between male and female crabs to this species.
Highlights
The stone violaceous crab Platyxanthus orbignyi [1] is an important resource for artisanal fisherman in the southeastern Pacific (Ecuador, Peru, and Chile) [2]
The levels of lipoperoxidation in hepatopancreas, gills, and hemolymph were evaluated in males and females of adult crabs Platyxanthus orbignyi, when they were exposed to air for 4 h, considering the transportation time from capture to market
The aim was to verify the physiological differences caused by increased levels of stress between both sexes that could modify the quality of the final product, mainly in gills and hepatopancreas, which are commonly consumed by the southern Pacific coastal population
Summary
The stone violaceous crab Platyxanthus orbignyi [1] is an important resource for artisanal fisherman in the southeastern Pacific (Ecuador, Peru, and Chile) [2]. Crabs are exposed to air for several hours in plastic containers until their arrival to the market, water flow through the gills is interrupted At this time an increase of reactive oxygen species (ROS) begins, producing oxidative stress, including lipid peroxidation (LPO) [4,5]. Different sexes of crabs could be able to present differences in their responses to stress, as shown in the species Callinectes amnicola and Geothelphusa dehaani, where males present greater oxidative damage and, as a consequence, experience a reduction in their population [13] In this brief work, the levels of lipoperoxidation in hepatopancreas, gills, and hemolymph were evaluated in males and females of adult crabs Platyxanthus orbignyi, when they were exposed to air for 4 h, considering the transportation time from capture to market. The aim was to verify the physiological differences caused by increased levels of stress between both sexes that could modify the quality of the final product, mainly in gills and hepatopancreas, which are commonly consumed by the southern Pacific coastal population
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