Abstract
AbstractThis paper reviews recent examples of the application of combined high temperature gas‐liquid chromatography (GLC) and reversed phase high pressure liquid chromatography (HPLC) with electron impact and chemical ionization mass spectrometry for structural studies of natural diacyl and triacylglycerols. It was concluded that the combination of reversed phase HPLC with direct liquid inlet chemical ionization mass spectrometry provides the most complete resolution and most reliable identification of natural acylglycerols, far exceeding the capabilities of either technique alone. The LC/MS method is suitable for quantitative analysis following appropriate calibration of the total or fragment ion response.
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