Lipid metabolism in juveniles of Yellowtail,Seriola dorsalis,fed different levels of dietary methionine containing a low level of cholesterol: Implication in feed formulation

Abstract

The involvement of dietary methionine in the lipid metabolism of Seriola dorsalis, at different levels of dietary methionine (Met), and low content of cholesterol was investigated. Four diets containing different Met levels and a low amount of cholesterol (0.23 g/kg diet) were prepared. One hundred and eighty juveniles (16.2 ± 1.5 g) were randomly distributed into each 12 500-L ponds. After 60 days, the growth in weight (%) significantly increased following the Met supplementation, whereas SGR of all the experimental treatments was higher than the Basal Diet. The expression of the insulin-like growth factor (IGF-1) increased significantly (0.2-fold) and was complemented by significant changes in Mat, BHMT and HMG-CoA, for fish that were in the low and higher Met levels. The cholesterol content in the whole body and liver increased following methionine supplementation, similarly to that observed for crude lipids, in particular in the whole body and liver tissues. However, the relative amount of most fatty acids remained unchanged. Only the oleic acid increased at a higher amount of Met. Therefore, once the dietary requirement of methionine is met, the methionine is used to synthesize cholesterol. It is recommended to supply a higher amount of methionine to spare energy for growth.