Abstract
Excessive lipid intake will cause hyperlipidemia, fatty liver metabolism disease, and endanger people’s health. Edible fungus polysaccharide is a natural active substance for lipid lowering. In this study, the HepG2 cell model induced by oleic acid and mice model induced by a high-fat diet was established. The lipid-lowering effects of Inonotus obliquus polysaccharide (IOP) was investigated in vivo and in vitro. Glucose (251.33 mg/g), rhamnose (11.53 mg/g), ribose (5.10 mg/g), glucuronic acid (6.30 mg/g), and galacturonic acid (2.95 mg/g) are present in IOP, at a ratio of 85.2:3.91:1.73:2.14:1. The molecular weight of IOP is 42.28 kDa. Treatment with 60 mg/L of IOP showed a significant lipid-lowering effect in HepG2 cells compared with the oleic acid-treated group. In the oil red O-stained images, the red fat droplets in the IOP-treated groups were significantly reduced. TC and TG levels of IOP-treated groups decreased. IOP can alleviate the lipid deposition in the mice liver due to high-fat diet, and significantly reduce their serum TC, TG, and LDL-C contents. IOP could activate AMPK but decrease the SREBP-1C, FAS, and ACC protein expression related to adipose synthesis in mice. IOP has a certain potential for lipid-lowering effects both in vivo and in vitro.
Highlights
With the improvement of people’s living standards, the intake of high-fat diets is increasing
The results showed that after the mice fed by highfat diet, mRNA levels of SREBP-1C, ACC, and fatty acid synthases (FAS) increased significantly, which was consistent with the result of increased lipid deposition in the mice liver
Inonotus obliquus polysaccharide (IOP) was a heteropolysaccharide with a molecular weight of 42.28 kDa
Summary
With the improvement of people’s living standards, the intake of high-fat diets is increasing. Excessive lipid intake can cause many metabolic diseases. Excessive release of saturated-fatty acids, refined carbohydrates, fructose, and high caloric intake in diets promote obesity and non-alcoholic fatty liver disease [1]. Excessive sugar intake, and oxidative damage are the main reasons of metabolic diseases [2]. Lipid accumulation is closely related to metabolic syndromes such as hypertension, hyperlipidemia, and non-alcoholic fatty liver disease, so it has attracted widespread attention [3]. Lipid metabolism in normal liver cells is in a balanced state, but when lipids accumulate too much, liver cells will experience steatosis. Reducing food intake, losing weight, and removing excessive fat surgically may alleviate lipid accumulation
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