Abstract
Summary: Several strains of Acetobacter xylinum were screened for in vivo cellulose and acetan production, and for in vitro synthesis of a prenyl-diphosphate-hexasaccharide, using UDP-Glc, UDP-GlcA and GDP-Man as sugar donors. The lipid-bound saccharide was synthesized only by acetan-producing strains. Previous work has shown that the in vitro-synthesized lipid-linked saccharides have the same structure as the acetan repeating unit. The present results strongly suggest a precursor-product relationship. The strains that produced acetan lost their ability to do so by ageing of the culture.
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