Abstract

ObjectiveThis study investigated the relationship between in-vitro lipid content in myotubes and in-vivo whole body phenotypes of the donors such as insulin sensitivity, intramyocellular lipids (IMCL), physical activity and oxidative capacity.Design and MethodsSix physically active donors were compared to 6 sedentary lean and 6 T2DM. Lipid content was measured in tissues and myotubes by immunohistochemistry. Ceramides, triacylglycerols (TAGs) and diacylglycerols (DAGs) were measured by LC-MS-MS and GC-FID. Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp (80mU/min/m2), maximal mitochondrial capacity (ATPmax) by 31P-MRS, physical fitness by VO2max and physical activity level (PAL) by accelerometers.ResultsMyotubes cultured from physically active donors had higher lipid content (0.047±0.003 vs. 0.032±0.001 and 0.033±0.001AU; p<0.001) than myotubes from lean and T2DM donors. Lipid content in myotubes was not associated with IMCL in muscle tissue but importantly, correlated with in-vivo measures of ATPmax (r=0.74; p<0.001), insulin sensitivity (r=0.54; p<0.05), type-I fibers (r=0.50; p<0.05) and PAL (r=0.92; p<0.0001). DAGs and ceramides in myotubes were inversely associated with insulin sensitivity (r=−0.55, r=−0.73; p<0.05) and ATPmax (r=−0.74, r=−0.85; p<0.01).ConclusionsThese results indicate that cultured human myotubes can be used in mechanistic studies to study the in vitro impact of interventions on phenotypes such as mitochondrial capacity, insulin sensitivity, and physical activity.

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