Abstract
The objective of this study was to understand the effect of biomass concentration (BC), milling times (MT) and volumetric hexane to water ratio (HWR) on lipid recovery rate of microalgae Scenedesmus dimorphus in the wet milling process. A second-order response surface regression model (R2>95%) was developed based on a three-variable five-level experiment using the Central Composite Design method. Modeling results indicated that HWR (Pr<0.001) and BC (Pr<0.01) significantly affected lipid recovery rate. The optimum settings were found to be 21.2 g l-1BC, 4 MT and 0.70 HWR, at which 98.5% of lipids were recovered. The results indicated that the wet grinding mill was effective in lipid recovery fromS. dimorphus.
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