Abstract
Lipid droplets (LDs) participate in many physiological processes, the abnormality of which will cause chronic diseases and pathologies such as diabetes and obesity. It is crucial to monitor the distribution of LDs at high spatial resolution and large depth. Herein, we carried three-photon imaging of LDs in fat liver. Owing to the large three-photon absorption cross-section of the luminogen named NAP-CF3 ([Formula: see text][Formula: see text]cm6 s2), three-photon fluorescence fat liver imaging reached the largest depth of 80[Formula: see text][Formula: see text]m. Fat liver diagnosis was successfully carried out with excellent performance, providing great potential for LDs-associated pathologies research.
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