Lipid domains in HIV-1 assembly.

Naresh Yandrapalli,Cyril Favard,Delphine Muriaux

doi:10.3389/fmicb.2014.00220

Abstract

In CD+4 T cells, HIV-1 buds from the host cell plasma membrane. The viral Gag polyprotein is mainly responsible for this process. However, the intimate interaction of Gag and lipids at the plasma membrane as well as its consequences, in terms of lipids lateral organization and virus assembly, is still under debate. In this review we propose to revisit the role of plasma membrane lipids in HIV-1 Gag targeting and assembly, at the light of lipid membranes biophysics and literature dealing with Gag-lipid interactions.

Highlights

In a very oversimplified view, assembling could be seen as: retrieving all of your partners at the right place on the right time
Gag has been reported to interact with a cellular motor protein (Tang et al, 1999; Martinez et al, 2008) and with other components of vesicular trafficking pathway (Dong et al, 2005; Camus et al, 2007), it is not clear whether Gag is targeted to the plasma membrane or reaches the plasma membrane by diffusion through the cytosol
It has been shown that Gag molecules do not multimerize extensively before they reach the membrane (Kutluay and Bieniasz, 2010) and that they arrive at the plasma membrane as dimers or monomers that will multimerize onto eventual nucleation sites composed of Gag-RNA complexes (Jouvenet et al, 2009; Ku et al, 2013)

Summary

Introduction

In a very oversimplified view, assembling could be seen as: retrieving all of your partners at the right place on the right time. Thereafter, the role of lipids in HIV-1 assembly will be considered, by looking at the interaction of Gag with the plasma membrane at the molecular (atomistic) level.

Results

Conclusion