Abstract
Phase coexistence between liquid phases in lipid membranes provides a ideal model system to investigate mixing in two dimensions. In this poster we will extend our previous comparison of passive observational techniques for line tension measurements in lipid monolayers to more complex systems. Using fluorescence microscopy and Langmuir troughs we will illustrate the role that experimental parameters (composition, transition kinetics, etc.) play in determining the size distribution for several lipid compositions. We also will utilize the novel two phase liquid-liquid coexistence regions of hydroxycholesterol-phospholipid lipid monolayers to test size distribution measurements of line tension.
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