Abstract
The distribution of different kinds of lipid was studied by histochemical and histophysical methods in human dental periapical lesions. After extraction of the implicated tooth, the lesion was separated from the root and subjected to special methods for detection of lipids. Staining with Sudan black B and with Nile blue sulfate and the use of acid hematein, osmium tetroxide, and birefringence were the techniques employed. Some methods for extracting lipids were used as controls. Different types of lipid were observed in the different stages of the lesions. Glycerides, free and esterified cholesterol, and phospholipids were found in these lesions. These lipids change quantitatively and qualitatively with the type of lesion.
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