Lipid-associated membrane lipopeptides of M. arginini activate NF-κB by interacting with TLR2/1, TLR2/6, and TLR2/CD14

Abstract

Various strains of mycoplasmas cause the activation of transcriptional factor NF-κB as a result of interactions with different combinations of Toll-like receptors (TLRs). It is well known that MALP-2 protein of M. fermentans activates NF-κB through interactions with TLR2/6, activates lipid-associated membrane lipopeptides (LAMPs) of M. penetrans through TLR1/2, activates LAMPs of M. pneumoniae through combinations of Toll-like receptors (TLR2/6 and TLR1/2), and activates the superantigene of M. arthritidis through TLR2- and TLR4-dependent pathways. In this study, we defined specific Toll-like receptors for LAMPs of M. arginini. For carrying out the research, we used the following cell lines, which express certain combinations of TLRs and their coreceptors: 293-null, 293-hTLR2, 293-hTLR1/2, 293-hTLR2/CD14, 293-hTLR2/6, and 293-hTLR4/CD14-MD2. It was shown that LAMPs of M. arginini cause the activation of NF-κB by interacting with TLR2/1, TLR2/6 and TLR2/CD14, but not with TLR2 or TLR4 alone.