Lipid and protein oxidation in newborn infants after lutein administration.

S Perrone,A Picardi,A Santacroce,M Longini,F Bazzini,M Tei,G Buonocore,C Felici,F Proietti,P Vasarri,G Turrisi

doi:10.1155/2014/781454

S Perrone, A Picardi + Show 9 more

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https://doi.org/10.1155/2014/781454

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Abstract

Objectives. To test the hypothesis that neonatal supplementation with lutein in the first hours of life reduces neonatal oxidative stress (OS) in the immediate postpartum period. Methods. A randomized controlled, double-blinded clinical trial was conducted among 150 newborns divided into control group, not supplemented (n = 47), and test group, supplemented with lutein on the first day postpartum (n = 103). Blood Samples were collected at birth from cord and at 48 hrs postpartum while routine neonatal metabolic screenings were taking place. Total hydroperoxide (TH), advanced oxidation protein products (AOPP), and biological antioxidant potential (BAP) were measured by spectrophotometry and data were analyzed by Wilcoxon rank sum test and by multivariate logistic regression analysis. Results. Before lutein supplementation, the mean blood concentrations of AOPP, TH, and BAP were 36.10 umol/L, 156.75 mmol/H2O2, and 2361.04 umol/L in the test group. After lutein supplementation, significantly higher BAP increment (0.17 ± 0.22 versus 0.06 versus ± 0.46) and lower TH increment (0.46 ± 0.54 versus 0.34 ± 0.52) were observed in the test group compared to controls. Conclusion. Neonatal supplementation with lutein in the first hours of life increases BAP and reduces TH in supplemented babies compared to those untreated. The generation of free radical-induced damage at birth is reduced by lutein. This trial is registered with ClinicalTrials.gov NCT02068807.

Highlights

Protecting the newborn infant against perinatal oxidative stress (OS) is an healthcare priority, and the search for new, safe, and efficacious antioxidants has been a major quest during the last decade.Among the therapeutic antioxidant approaches, lutein, a compound belonging to the xanthophyll family of carotenoids, is one of the emerging strategies applied in newborns
In a preliminary pilot study we found that lutein supplementation to newborns infants in the first days of life reduced free radical formation and oxidative injury [20]
biological antioxidant potential (BAP) test is based on the ability of colored solution, containing ferric (Fe3+) ions adequately bound to special chromogenic substrate, to decolor when its Fe3+ ions are reduced to ferrous (Fe2+) ions and it can be observed by adding a reducing system, that is, blood plasma as well

Summary

Introduction

Protecting the newborn infant against perinatal oxidative stress (OS) is an healthcare priority, and the search for new, safe, and efficacious antioxidants has been a major quest during the last decade.Among the therapeutic antioxidant approaches, lutein, a compound belonging to the xanthophyll family of carotenoids, is one of the emerging strategies applied in newborns. Previous experimental reports demonstrated that lutein has antiangiogenic and neuroprotective properties [5, 6] and studies in vitro proved its protective effect on macula and photoreceptors against phototoxicity and oxidative injury [7, 8]. This compound is able to ameliorate in vitro and in vivo inflammatory responses by suppressing nuclear factor kappa B (NF-κB) activation [9, 10]. These findings support the role of lutein in modulating inflammatory processes by regulating cellular redox potential

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Conclusion