Abstract
AbstractMost lipid extracts are a mixture of saturated and unsaturated molecules. Therefore, the most successful high performance liquid chromatography (HPLC) detectors for the quantitative analysis of lipids have involved the use of “universal” or “mass” detectors such as flame ionization detectors and evaporative light scattering detectors. Recently a new type of HPLC “universal” detector, a charged aerosol detector (CAD), was developed and is now commercially available. This detection method involves nebulizing the HPLC column effluent, evaporating the solvents, charging the aerosol particles and measuring the current from the charged aerosol flux. During the approximately four years that the charged aerosol detector has been commercially available, several publications have described HPLC‐CAD methods for lipid analysis. The most common lipids can be quantitatively analyzed via HPLC‐CAD except for some volatile lipids such as common fatty acid methyl esters and short chain free fatty acids (<C16). The major results of these publications will be summarized in this report.
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