Abstract
Large amount of palm oil mill effluent (POME) is generated annually. The waste would bepotential for production of single cell oils (SCOs). The objective of this study was to evaluatelipid accumulation by fungi using POME as substrate. Seven filamentous fungi were initiallyisolated from various biomes. The study results showed that out of these 7 fungi, five of themproduced endoglucanase and accumulated lipid about 34.3-87.5 persen of their dry cell mass usingPOME as substrate. The five fungi were identified as ATH, sp.,sp., sp. 1 T30, and sp.2 T50. The highest lipid accumulation wasobtained by ATH. The profile of trans-esterified SCOs revealed high content ofsaturated and mono-unsaturated fatty acids i.e., palmitic (C16:0), stearic (C18:0) and oleic(C18:1) acids similar to conventional vegetable oils used for biodiesel production. The strainthat was able to use organic substrates in POME indicated that they are promising strain forbiofuel feedstock as well as for fulfilling effluent quality for wastewater discharge BIOTROPIA Vol. 21 No. 2, 2014: 100 - 110
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