Abstract
BackgroundMaonan nationality is a relatively conservative and isolated minority in the Southwest of China. Little is known about the association of endothelial lipase gene (LIPG) single nucleotide polymorphisms (SNPs) and serum lipid levels in the Chinese populations.MethodsA total of 1280 subjects of Maonan nationality and 1218 participants of Han nationality were randomly selected from our previous stratified randomized samples. Genotypes of the four LIPG SNPs were determined by polymerase chain reaction-restriction fragment length polymorphism, and then confirmed by direct sequencing.ResultsSeveral SNPs were associated with high-density lipoprotein cholesterol (rs3813082, rs2000813 and rs2097055) in the both ethnic groups; total cholesterol and apolipoprotein (Apo) A1 (rs2000813) in Han nationality; and low-density lipoprotein cholesterol, ApoB, triglyceride (rs2097055) and ApoA1 (rs3819166) in Maonan minority (P < 0.0125 for all after Bonferroni correction). The commonest haplotype was rs3813082T-rs2000813C-rs2097055T-rs3819166A (Han, 44.2% and Maonan, 48.7%). The frequencies of the T-C-T-A, T-C-T-G, T-T-C-G and G-T-C-G haplotypes were different between the Maonan and Han populations (P < 0.05–0.001). The associations between haplotypes and dyslipidemia were also different in the Han and/or Maonan populations (P < 0.05–0.001).ConclusionsThe differences in serum lipid profiles between the two ethnic groups might partly be attributed to these LIPG SNPs, their haplotypes and gene-environmental interactions.Trial registrationRetrospectively registered.
Highlights
Maonan nationality is a relatively conservative and isolated minority in the Southwest of China
Genotypic frequencies of the lipase gene (LIPG) rs3813082 Single nucleotide polymorphism (SNP) were significantly different between the Maonan and Han ethnic groups (P = 1.4E-04, P < 0.0125 was considered statistically significant after the Bonferroni correction); and the allelic frequencies of the four LIPG SNPs were significantly different between the Maonan and Han populations (P < 0.05–0.01; Tables 3 and 4)
The present study shows that the genotypic frequencies of the rs3813082 SNP and the allelic frequencies of the four SNPs were different between the two ethnic groups
Summary
Maonan nationality is a relatively conservative and isolated minority in the Southwest of China. Little is known about the association of endothelial lipase gene (LIPG) single nucleotide polymorphisms (SNPs) and serum lipid levels in the Chinese populations. Cardiovascular disease (CVD) is a major cause of morbidity and mortality in both developed and developing countries in the world [1]. The mortality for this condition has gradually declined over the last decades in western countries [2], it is still the second leading cause of death in China [3, 4]. Human endothelial lipase gene (LIPG, as known as EL, EDL, PRO719; Gene ID: 9388; HGNC ID: 6623), which maps to chromosome 18q12.1–q12.3, spans 30 kb with 10 exons and encodes a polypeptide of 500 amino acids. LIPG belongs to the triglyceride (TG) lipase gene family with 46% identity to lipase member H (LIPH), 45% to lipoprotein lipase (LPL), 40% to hepatic lipase (HL) and 31% to pancreatic lipase (PNLIP) [11,12,13].
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