Abstract
Hormone sensitive lipase is one of three enzymes involved in lipolysis process and encoded by LIPE gene. In this study we investigated LIPE gene polymorphism c.442 G>A influence on carcass traits in hybrid pigs. Genomic DNA extracted using Chelex resin, genotypes determined using RFLP-PCR. Allele A observed with frequency 0,738, allele G - 0,262. The most common genotype was AA, genotype GG was observed with lower frequency, genotype AG was rarest. While evaluating population heterozygosity, it was noticed that observed heterozygosity was only 0,075, while expected heterozygosity was 0,387. In observed pig population allele A is associated with better animal muscularity, allele G - with greater fat content.
Highlights
According to the United Nations Food and Agriculture Organization pork is the most consumed meat per capita in the world
Modifications in gene sequence can lead to altered hormone-sensitive lipase (HSL) function, which can induce incomplete lipolysis and fat accumulation in body
After inheritance analysis both alleles (A, G) were observed in Meishan pigs, in Pietrain pigs G allele was fixated, Landrace and Large White breeds were monomorphic to allele G, Duroc breed was polymorphic (Knoll et al, 1998)
Summary
According to the United Nations Food and Agriculture Organization pork is the most consumed meat per capita in the world. It is very important to know about the quality of consumed meat, which can be determined by animal’s age, breed, genetic or environmental factors. Ongoing pig selection in the world is based on lowering fat content in the meat and increasing lean carcass yield (Zimmermann et al 2004; Tyra et al 2011). To maintain valuable meat traits in all generations pig selection should be based on genetic, not phenotypical properties. A lot of research work was done to evaluate genes candidates and their influence on carcass traits. Selected genes candidates are carefully evaluated in various animal populations, accurate enzyme function is determined, polymorphism influence on phenotypic traits is evaluated. Selected genes are called markers; they are included in quantitative trait locus (QTL) maps
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
