Abstract
An enzymatic synthesis of sugar ester from 1,2- o-isopropylidene- d-xylofuranose and fatty acid was studied. Optimal conditions for ester synthesis catalyzed by lipase were established. Lipases N-435 and IM resulted in the highest extent of esterification. The optimum temperature for the sugar ester synthesis reaction by lipase N-435 was 60°C. When the arachidonic acid to 1,2- o-isopropylidene- d-xylofuranose ratio was 1–2:1, a maximum conversion of 83–85% was obtained. After the reaction, the enzyme was easily separated and recovered for reuse. The chemical structure of the sugar ester was confirmed to be xylose 5-arachidonate by nuclear magnetic resonance and mass spectrometry.
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