Abstract
The development of a biotechnological method for the production of new biologically active phosphatidylcholine containing monoterpene citronellic acid (CA) was the aim of this work. Incorporation of citronellic acid (CA) into egg-yolk phosphatidylcholine (PC) in the lipase-catalyzed acidolysis process was studied. Isoprenoid acid CA was used as an acyl donor and five commercially available immobilized lipases were examined as biocatalysts. The effects of organic solvent, enzyme load, reaction time and molar ratio of substrates on the incorporation of citronellic acid (CA) into the phospholipids were evaluated. Modified phospholipid fraction enriched with CA in the sn-1 position (39% of incorporation) was obtained in high 33% yield using Novozym 435 as biocatalyst. In this study a biotechnological method for production of new phospholipid biopreparation enriched with citronellic acid, which can play an important role as a nutraceutical, was applied.
Highlights
Isoprenoids are a wide group of natural compounds occurring, both in vegetal and animal species, where they play important biological functions such as pheromones, phytoalexins and antifeedants [1,2]
In the production of structured phospholipids with the defined fatty acids profile, having biological applications, the enzyme-catalyzed reactions have the decisive advantage over chemical
In the production of structured phospholipids with the defined fatty acids profile, having biological applications, the enzyme-catalyzed reactions have the decisive advantage over chemical reactions
Summary
Isoprenoids are a wide group of natural compounds occurring, both in vegetal and animal species, where they play important biological functions such as pheromones, phytoalexins and antifeedants [1,2]. Perillyl alcohol, which is in the second phase of clinical trials exhibits significant ability to induce apoptosis of prostate cancer cells either in in vitro and in in vivo tests [10]. Another well-established chemopreventive and therapeutic agent against many cancer cell lines being under the clinical trial (phase I and phase II) is limonene [7]. Acyclic isoprenoids such as geraniol, farnezol or geranylogeraniol exhibit antiproliferative activity towards selected tumor cell lines as well [11,12,13]
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