Abstract
Nitrogen and carbon sources influencing the growth and production of lipase by Rhizopus oryzae were studied. High yields of enzyme activity were obtained when proteose peptone was the nitrogen source in media with olive oil (MWO) and without olive oil (MWHO). Carbon sources increased lipase activity in MWHO but decreased it slightly in the presence of oil. Lipase activity was significantly (P < 0.5) higher in MWO than in MWHO. Biomass concentration was also higher in the presence of oil. Industrial by-products bran and whey have been used as efficient media for the production of lipase by R oryzae. © 1999 Society of Chemical Industry
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