Abstract
AbstractEngineering media for optimal product yield in enzyme‐catalyzed reactions is an important strategy. We report here synthesis of dodecyl oleate and oleyl oleate by lipase (Candida rugosa) in solvent‐free substrate foams. Ester formation was characterized with respect to enzyme concentration, pH, temperature, and substrate concentration. The kinetics of ester formation suggest that the formation of ester was 80% complete in 2h. The pH and temperature optima of lipase suggest that the behavior of lipase in substrate foams was similar to its behavior in water or in organic solvents. The denaturing effect of foams on enzyme was evaluated. Rapid loss in activity (>70% in 1 h) was observed in the presence of oleic acid and dodecanol. The large surface areas generated in aqueous foams offer better accessibility of substrate to lipase for esterification.
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