Abstract
Dietary oil spillage has become a widespread and increasing threat to the environment worldwide. Lipase produced by bacteria provides an efficient, environment-friendly and cost-saving way to degrade dietary oil waste. Thus, selection of highly efficient lipases becomes an urgent need to solve dietary oil-related environmental issues. In this study, with comparison analysis of different bacteria species showing oil degradation abilities, we found Bacillus cereus XN12 displayed higher dietary oil degradation efficiency than Bacillus subtilis and Enterobacter cloacae. Bacillus cereus flourishes better in oil-containing medium than non-oil medium, which indicated that Bacillus cereus prefers to use dietary oil as their energy source. We then successfully cloned the lipase gene from Bacillus cereus XN12 and expressed this Bacillus cereus XN12 lipase gene in Escherichia coli BL21. The Escherichia coli expressing the recombinant Bacillus cereus lipase protein displayed significant higher lipase activity and oil degradation ability. These results suggested that lipase from Bacillus cereus XN12 provides a potential solution to alleviate dietary oil pollution.
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