Abstract
This work investigated the immobilised lipase kinetics of esterification of oleic acid and ethanol. The reaction was conducted under supercritical conditions (13 × 106 Pa and 40 °C) using carbon dioxide as solvent in a continuous packed bed (plug flow) reactor. Biocatalyst LypozymeTM IM60, which is lipase from Rhizomucor miehei (EC.3.1.1.3), immobilised on Duolite (anionic exchange resin) was used as biocatalyst. Kinetically, with regard to oleic acid, the reaction was successfully modelled by the Michaelis–Menten mechanism. The reaction rate constants Km and Vmax were evaluated. Furthermore, it was found to undergo competitive inhibition by ethanol, and the inhibition constant Ki was evaluated. © 2000 Society of Chemical Industry
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