Linking isotopic and migratory patterns in a pelagic seabird

Abstract

The value of stable isotope analysis in tracking animal migrations in marine environments is poorly understood, mainly due to insufficient knowledge of isotopic integration into animal tissues within distinct water masses. We investigated isotopic and moult patterns in Cory's shearwaters to assess the integration of different stable isotopes into feathers in relation to the birds' transoceanic movements. Specimens of Mediterranean Cory's shearwater Calonectris diomedea diomedea caught accidentally by Catalan longliners were collected and the signatures of stable isotopes of C (delta(13)C), N (delta(15)N) and S (delta(34)S) were analysed in 11 wing and two tail feathers from 20 birds, and in some breast feathers. Based on isotopic signatures and moult patterns, the feathers segregated into two groups (breeding and wintering), corresponding to those grown in the Mediterranean or Atlantic regions, respectively. In addition, feathers grown during winter, i.e. moulted in Atlantic waters, were grouped into two isotopically distinct profiles, presumably corresponding to the two main wintering areas previously identified for Mediterranean Cory's shearwater in tracking studies. N signatures mainly indicated the Mediterranean-to-Atlantic migration, whereas C and S signatures differed according to the Atlantic wintering area. Our results indicate that isotopic signatures from distant oceanic regions can integrate the feathers of a given bird and can indicate the region in which each feather was grown. This study thus underscores how stable isotope analysis can link marine animals to specific breeding and wintering areas, and thereby shed new light on studies involving assignment, migratory connectivity and carry-over effects in the marine environment.