Linkages of nitrogen-cycling microbial resistance and resilience to soil nutrient stoichiometry under dry-rewetting cycles with different fertilizations and temperatures in a vegetable field

Abstract

Multiple dry-rewetting (DRW) cycles occur in intensively managed vegetable fields due to frequent tillage and irrigation. Soil nitrogen (N) cycling depends on the resistance and resilience of related microbial populations to DRW cycles, which could be closely related to soil nutrient status. However, the linkage of N-cycling microbial resistance and resilience and soil nutrient stoichiometry remains unknown in vegetable field. Here, we established four fertilization treatments in a four-year greenhouse vegetable field: no N fertilization, synthesized N fertilization, substituting 50% of chemical N with organic fertilizer or biofertilizer. Then, we set up an 85-day DRW-cycling incubation at 15, 25 and 35 °C including a 55-day fluctuating moisture for microbial resistance and then a 30-day constant moisture for microbial resilience. The results showed that microbial resistance was high (resistance index = 0.87– 0.99) in response to DRW cycles, but microbial resilience was generally low (resilience index = −0.36– 0.76), especially in 50% organic substitution or 15 °C. N-cycling microbes showed an important trade-off between their resistance and resilience to DRW cycles. Furthermore, most treatments showed microbial carbon limitation and N abundance during DRW cycles and recovered gradually to the undisturbed state. Microbial resistance was significantly related to the soil nutrient stoichiometry of carbon, N and phosphorus, while microbial resilience was mainly correlated with carbon-related indicators. In conclusion, N-cycling microbes presented good stability with oligotrophic strategy to frequent DRW cycles, which was linked to not only the historical legacy effect of DRW cycles but also soil nutrient stoichiometry in the vegetable field.