Abstract
plasmid vectors. The probes pBLT6, pBLT42, and pBLT73 are in vector pIBI30 (International Biotechnologies Inc.) while pBLT68 is in vector pUC18 (Yanisch-Perron et al. 1985). Plasmid DNA was digested with a restriction endonuclease to release the insert. DNA fragments were purified on agarose gels. The DNA fragments used as probes were radiolabeled using the random oligonucleotide labeling procedure of Feinberg and Vogelstein (1983). Random hexamers [pd(N)6 ] were purchased from Pharmaeia (Piscataway, N.J.). Appro- ximately 50 ng of DNA was used per 25 gl of reaction with 50 gCi of [e-32p]-deoxycytidine 5'-triphosphate (3000 Ci/mmol) (NEN/DuPont, Wilmington, DeL). Reactions were incubated at room temperature for 2-3 h. Plant analysis A comprehensive genetic map of soybean would be very valuable to soybean breeders and geneticists in devising breeding strategies for soybean improvement. A rudimentary classical genetic map has been developed with great effort over many years. This map needs to be integrated with the molecular map now emerging from current research in order to use the molecular map as a bridge to obtain a comprehensive map of the organismically important genes. In the present study, we tested the as yet unmapped
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